6AGO
Crystal structure of MRG15-ASH1L Histone methyltransferase complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-18 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 115.757, 115.757, 209.282 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.917 - 3.103 |
R-factor | 0.2235 |
Rwork | 0.220 |
R-free | 0.27230 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
Rmerge | 0.064 | 0.033 | 0.736 |
Rmeas | 0.071 | 0.037 | 0.830 |
Rpim | 0.030 | 0.016 | 0.373 |
Total number of observations | 147885 | ||
Number of reflections | 29386 | 1554 | 1461 |
<I/σ(I)> | 8 | ||
Completeness [%] | 99.9 | 99.6 | 99.9 |
Redundancy | 5 | 4.8 | 4.6 |
CC(1/2) | 0.998 | 0.814 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 295 | Ammonium Acetate |