6AGM
Molecular basis for feedback inhibition of tyrosine-regulated 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.189, 133.859, 87.670 |
Unit cell angles | 90.00, 91.23, 90.00 |
Refinement procedure
Resolution | 38.207 - 2.000 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qr7 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.737 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rpim | 0.036 | 0.368 |
Number of reflections | 82353 | 6902 |
<I/σ(I)> | 19.4 | 1 |
Completeness [%] | 97.0 | |
Redundancy | 6.4 | |
CC(1/2) | 0.997 | 0.651 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 16% PEG 4000, 10% 2-propanol, 0.1 M sodium citrate, pH 5.5 |