6AG5

Crystal structure of Ard1 N-terminal acetyltransferase E88H/H127E mutant from Sulfolobus solfataricus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	77
Detector technology	CCD
Collection date	2013-12-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9765
Spacegroup name	P 21 21 21
Unit cell lengths	41.217, 52.926, 73.576
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.314 - 2.320
R-factor	0.1691
Rwork	0.160
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3k
RMSD bond length	0.008
RMSD bond angle	1.194
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.320
High resolution limit [Å]	2.320	2.300
Number of reflections	7313
<I/σ(I)>	10.69
Completeness [%]	99.4
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	283	20% Tacsimate pH 7.0