Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 116.290, 116.290, 234.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.030 - 2.100 |
| R-factor | 0.18793 |
| Rwork | 0.186 |
| R-free | 0.21665 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j49 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.102 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.030 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.171 | 0.831 |
| Rpim | 0.046 | 0.229 |
| Number of reflections | 94385 | 13597 |
| <I/σ(I)> | 9.7 | 3.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.5 | 13.7 |
| CC(1/2) | 0.996 | 0.908 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100 mM MES-Na (pH 6.0) and 1.26 M ammonium sulfate |
