6A8B
Ribokinase from Leishmania donovani with AMPPCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-28 |
| Detector | RIGAKU AFC-5R |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 61 |
| Unit cell lengths | 100.395, 100.395, 125.785 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.790 - 2.010 |
| R-factor | 0.18604 |
| Rwork | 0.184 |
| R-free | 0.22592 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6a8a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.676 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.790 | 2.060 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.113 | 0.661 |
| Number of reflections | 47985 | 3514 |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 296 | 0.1M Citric acid pH 4.3, 3.4M Sodium chloride, 10mM Magnesium chloride, 10% Glycerol |
