6A45
Structure of mouse TREX2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.293, 54.725, 88.156 |
| Unit cell angles | 90.00, 100.70, 90.00 |
Refinement procedure
| Resolution | 26.427 - 1.904 |
| R-factor | 0.1857 |
| Rwork | 0.183 |
| R-free | 0.21970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y97 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.990 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.067 | 0.383 |
| Rmeas | 0.078 | 0.458 |
| Rpim | 0.039 | 0.246 |
| Number of reflections | 32343 | 3127 |
| <I/σ(I)> | 18.85 | 1.76 |
| Completeness [%] | 99.2 | 97.3 |
| Redundancy | 3.8 | 3 |
| CC(1/2) | 0.776 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES sodium pH 7.5, 0.8M Sodium phosphate monobasic monohydrate, 0.8M Potassium phosphate monobasic |
