6A3A
MVM NES mutant Nm2 in complex with CRM1-Ran-RanBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 106.650, 106.650, 304.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.990 - 2.300 |
| R-factor | 0.2111 |
| Rwork | 0.209 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hat |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.942 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
| Rmerge | 0.119 | 0.065 | |
| Rmeas | 0.125 | 0.068 | |
| Rpim | 0.045 | 0.020 | 0.867 |
| Total number of observations | 972120 | ||
| Number of reflections | 78675 | 4344 | 3843 |
| <I/σ(I)> | 2.8 | ||
| Completeness [%] | 99.6 | 99.9 | 99 |
| Redundancy | 12.4 | 11.7 | 12.7 |
| CC(1/2) | 0.997 | 0.330 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 18% PEG3350, 200mM Ammonium Nitrate, 100mM Bis-Tris pH6.6 |
