6A32

Crystal structure of PDGFRA kinase domain mutant T674I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9777
Spacegroup name	P 21 21 21
Unit cell lengths	37.194, 87.604, 96.332
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.698 - 1.870
R-factor	0.2055
Rwork	0.204
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k5x
RMSD bond length	0.011
RMSD bond angle	1.167
Data reduction software	DENZO
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.870	1.870
Number of reflections	26260
<I/σ(I)>	15.8
Completeness [%]	99.9
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Bis-Tris pH 5.5, 27% PEG 3350