6A1Z

Crystal Structure of dimeric Kinesin-3 KIF13B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9789
Spacegroup name	P 32 2 1
Unit cell lengths	87.380, 87.380, 97.490
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.869 - 2.580
R-factor	0.2401
Rwork	0.238
R-free	0.28640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zbr
RMSD bond length	0.009
RMSD bond angle	1.119
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.870	2.650
High resolution limit [Å]	2.580	2.580
Rmerge	0.062	0.665
Number of reflections	13889	1023
<I/σ(I)>	14
Completeness [%]	99.4
Redundancy	18.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.9	289	1.4M Na/KPO4