6A1C

Crystal structure of the CK2a1-go289 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	95
Detector technology	CCD
Collection date	2015-04-18
Detector	RAYONIX MX300HE
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	51.657, 78.831, 79.403
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.940 - 1.680
R-factor	0.14042
Rwork	0.138
R-free	0.19627
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3war
RMSD bond length	0.018
RMSD bond angle	1.921
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.950	1.710
High resolution limit [Å]	1.680	1.680
Rmerge	0.056	0.462
Number of reflections	37267
<I/σ(I)>	30.6	5.7
Completeness [%]	99.6	99.3
Redundancy	7.3	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	ethyleneglycol