6A10

Crystal structure of hen egg white lysozyme crystallized by ammonium sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-19
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	41.399, 57.971, 57.618
Unit cell angles	115.97, 105.20, 98.81

Refinement procedure

Resolution	19.295 - 1.130
R-factor	0.164
Rwork	0.163
R-free	0.17610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wld
RMSD bond length	0.011
RMSD bond angle	1.365
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.150
High resolution limit [Å]	1.130	3.070	1.130
Rmerge	0.042	0.022	0.359
Rmeas	0.060	0.032	0.508
Rpim	0.042	0.022	0.359
Total number of observations	293247
Number of reflections	151134	5732	7388
<I/σ(I)>	12.8
Completeness [%]	91.4	69.4	89.1
Redundancy	1.9	1.9	1.9
CC(1/2)		0.995	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	1.2M ammonium sulfate, 6%(v/v) 1,4-dioxane