5ZZ6

Redox-sensing transcriptional repressor Rex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97940
Spacegroup name	P 1 21 1
Unit cell lengths	53.512, 89.454, 87.025
Unit cell angles	90.00, 96.76, 90.00

Refinement procedure

Resolution	29.917 - 2.200
R-factor	0.2084
Rwork	0.206
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.665
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge		0.448
Number of reflections	41917
<I/σ(I)>	16.57
Completeness [%]	99.3
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	potassium chloride, PEG 3350