5ZYT
Crystal structure of human MGME1 with 3' overhang double strand DNA3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9725 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.497, 77.489, 164.714 |
| Unit cell angles | 90.00, 112.40, 90.00 |
Refinement procedure
| Resolution | 29.957 - 2.702 |
| R-factor | 0.2519 |
| Rwork | 0.249 |
| R-free | 0.29620 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.094 | |
| Number of reflections | 44359 | 4221 |
| <I/σ(I)> | 15.4 | |
| Completeness [%] | 94.6 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.684 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1 M HEPES pH 7.5, 0.8M NaH2PO4, and 0.8M K2HPO4. |
