5ZWT
Crystal structure of the S37A mutant of apo-acyl carrier protein from Leishmania major
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-27 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.979490 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 83.506, 83.506, 99.584 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.993 - 2.000 |
R-factor | 0.2362 |
Rwork | 0.234 |
R-free | 0.27210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1x3o |
RMSD bond length | 0.007 |
RMSD bond angle | 0.877 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.750 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.065 | 0.956 |
Rpim | 0.021 | 0.303 |
Number of reflections | 11845 | 886 |
<I/σ(I)> | 21.7 | |
Completeness [%] | 96.7 | 100 |
Redundancy | 10.6 | 10.9 |
CC(1/2) | 1.000 | 0.819 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 294 | 0.1M HEPES, pH 7.5, 2.0M ammonium sulphate |