5ZVB
APOBEC3F Chimeric Catalytic Domain in Complex with DNA(dT9)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-06 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.990, 92.702, 93.674 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.000 |
R-factor | 0.1742 |
Rwork | 0.172 |
R-free | 0.22030 |
Structure solution method | SAD |
Data reduction software | ADDREF |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 2.020 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.146 | 0.662 |
Number of reflections | 37457 | |
<I/σ(I)> | 5.3 | |
Completeness [%] | 99.4 | |
Redundancy | 14.3 | |
CC(1/2) | 0.999 | 0.936 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.05M sodium cacodylate, pH 6.5, and 30%(v/v) 2-methyl-2,4-pentanediol (MPD) |