5ZUI

Crystal Structure of HSP104 from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2015-06-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 65
Unit cell lengths	131.182, 131.182, 137.042
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.382 - 2.701
R-factor	0.214
Rwork	0.211
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ciu
RMSD bond angle	0.770
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	36348
<I/σ(I)>	31.6	1.2
Completeness [%]	99.1	93.1
Redundancy	10.1	5.1
CC(1/2)	0.998	0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.01 M Cobalt chloride, 0.1 M MES (pH6.5), 1.8 M Ammonium sulfate