5ZQV
Crystal Structure of Protein Phosphate 1 complexed with PP1 binding domain of GM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 41 |
Unit cell lengths | 111.973, 111.973, 195.299 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.288 - 2.950 |
R-factor | 0.2537 |
Rwork | 0.252 |
R-free | 0.29670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mp0 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.567 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
High resolution limit [Å] | 2.950 | 8.000 | 2.950 |
Rmerge | 0.099 | 0.075 | 1.336 |
Rpim | 0.096 | ||
Number of reflections | 50718 | 2411 | 2533 |
<I/σ(I)> | 3.2 | ||
Completeness [%] | 99.5 | 92.2 | 100 |
Redundancy | 7.4 | 6.9 | 7.4 |
CC(1/2) | 0.995 | 0.608 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.2M lithium citrate, 11% poly(ethylene glycol) 3350, 4.5% benzamidine hydrochloride hydrate |