5ZQT
Crystal structure of Oryza sativa hexokinase 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 131.600, 131.600, 188.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.790 - 2.840 |
| R-factor | 0.20638 |
| Rwork | 0.203 |
| R-free | 0.26590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qs7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.188 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.790 | 2.910 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.073 | 0.535 |
| Number of reflections | 45291 | 3318 |
| <I/σ(I)> | 30.56 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.1 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M MOPSO, 0.1 M Bis-Tris, 5%w/v PEG20000, 25% w/v Trimethypropane, 1% w/v NDSB195, 0.1 M Spermine tetrahydrochloride, 0.1 M Spermidine trihydrochloride, 0.1 M 1,4-Diaminobutane dihydrochloride, 0.1 M DL-Ornithine monohydrochloride |
