5ZQ8
Crystal structure of spRlmCD with U747 stemloop RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9774 |
Spacegroup name | P 1 |
Unit cell lengths | 62.230, 62.259, 79.818 |
Unit cell angles | 74.58, 85.47, 65.03 |
Refinement procedure
Resolution | 56.370 - 2.180 |
R-factor | 0.248 |
Rwork | 0.245 |
R-free | 0.30330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xj1 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.318 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 56.370 | 56.370 | 2.290 |
High resolution limit [Å] | 2.180 | 6.880 | 2.180 |
Rmerge | 0.130 | 0.081 | 0.551 |
Rmeas | 0.152 | 0.095 | 0.647 |
Rpim | 0.078 | 0.049 | 0.338 |
Total number of observations | 176570 | 6122 | 19299 |
Number of reflections | 48209 | 1721 | 5469 |
<I/σ(I)> | 6.2 | 11.6 | 2.2 |
Completeness [%] | 87.8 | 98.6 | 67.9 |
Redundancy | 3.7 | 3.6 | 3.5 |
CC(1/2) | 0.991 | 0.990 | 0.891 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M Ammonium acetate, 0.1M sodium acetate, pH5.2, 20%(w/v) PEG3350 |