5ZPU

LFS829 in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	105.438, 105.438, 303.907
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.600
R-factor	0.2262
Rwork	0.225
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hat
RMSD bond length	0.008
RMSD bond angle	1.255
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.010	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.313	0.167
Rmeas	0.306	0.169
Rpim	0.059	0.025	0.230
Number of reflections	54140	3018	2661
<I/σ(I)>	2.1
Completeness [%]	100.0	100	100
Redundancy	42.5	44.8	39.2
CC(1/2)		0.997	0.421

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	18% PEG3350, 200mM Ammonium Nitrate, 100mM Bis-Tris pH6.6