5ZOH
Crystal structure of a far-red light-absorbing form of AnPixJg2_BV4 in complex with biliverdin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-25 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.957, 60.650, 55.475 |
| Unit cell angles | 90.00, 108.59, 90.00 |
Refinement procedure
| Resolution | 31.670 - 1.600 |
| R-factor | 0.20785 |
| Rwork | 0.206 |
| R-free | 0.24075 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w2z |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.083 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.041 | 0.388 |
| Number of reflections | 27039 | 2737 |
| <I/σ(I)> | 28.2 | 3.1 |
| Completeness [%] | 98.0 | 99.9 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | 0.1 M sodium cacodylate, 26% PEG 3350, 0.2 M ammonium sulfate |
