5ZLP

Crystal structure of glutamine synthetase from helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-31
Detector	ADSC QUANTUM 315
Wavelength(s)	0.95950
Spacegroup name	C 1 2 1
Unit cell lengths	234.409, 135.167, 203.080
Unit cell angles	90.00, 91.61, 90.00

Refinement procedure

Resolution	47.264 - 2.930
R-factor	0.1716
Rwork	0.168
R-free	0.24610
RMSD bond length	0.011
RMSD bond angle	1.172
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.900	7.860	2.900
Rmerge	0.148	0.086	0.367
Rmeas	0.177	0.105	0.434
Rpim	0.096	0.059	0.231
Total number of observations	454812
Number of reflections	135884	6709	6838
<I/σ(I)>	8.8
Completeness [%]	98.8	95.1	99.7
Redundancy	3.3	3	3.4
CC(1/2)		0.982	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	296	sodium citrate, cosium formate