5ZK9
Stapled-peptides tailored against initiation of translation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.983994 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.420, 38.080, 78.490 |
| Unit cell angles | 90.00, 122.72, 90.00 |
Refinement procedure
| Resolution | 45.650 - 1.760 |
| R-factor | 0.17881 |
| Rwork | 0.177 |
| R-free | 0.21150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tpw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.684 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 22709 | 3574 |
| <I/σ(I)> | 15.33 | 2.18 |
| Completeness [%] | 98.6 | |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 12.5% pEG1000 w/v, 12.5% PEG3350 w/v, 12.5% MPD w/v, 0.1M MES/imidazole pH 6.5 |
