5ZI4
MDH3 wild type, nad-oaa-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-02-03 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.218, 86.658, 135.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.750 - 2.100 |
| R-factor | 0.19911 |
| Rwork | 0.196 |
| R-free | 0.26591 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mld |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.910 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rpim | 0.028 | 0.186 |
| Number of reflections | 40043 | |
| <I/σ(I)> | 40 | 5.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% (w/v) PEG 10000, 0.1M HEPES pH 7.5 |
