5ZHQ
Crystal structure of Scylla paramamosain arginine kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 190 |
| Detector technology | PIXEL |
| Collection date | 2017-11-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 136.720, 136.720, 145.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.969 - 3.002 |
| R-factor | 0.1832 |
| Rwork | 0.178 |
| R-free | 0.22790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4am1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.839 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmeas | 0.073 | |
| Number of reflections | 30704 | 2292 |
| <I/σ(I)> | 18.35 | 1.28 |
| Completeness [%] | 96.0 | 97.1 |
| Redundancy | 5 | 5 |
| CC(1/2) | 0.999 | 0.559 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 2.5 M Ammonium Sulfate, 0.1 M MES pH 6.0 |
