5ZF1

Molecular structure of a novel 5,10-methylenetetrahydrofolate dehydrogenase from the silkworm, Bombyx mori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-15
Detector	AGILENT EOS CCD
Wavelength(s)	0.9
Spacegroup name	P 43
Unit cell lengths	89.789, 89.789, 127.306
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.295 - 1.750
R-factor	0.2127
Rwork	0.211
R-free	0.24090
RMSD bond length	0.007
RMSD bond angle	0.869
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.300	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.057	0.487
Number of reflections	100729
<I/σ(I)>	52.3
Completeness [%]	99.3
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M ammonium sulfate, 0.1 M sodium cacodylate trihydrate, 30% PEG 8000