5ZEN

Crystal structure of human topoisomerase II beta in complex with DNA: a new quaternary conformation showing opening of the protein-linked DNA-gate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	95.760, 95.760, 231.055
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.970 - 2.750
R-factor	0.224
Rwork	0.221
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qx3
RMSD bond length	0.007
RMSD bond angle	0.782
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.850
High resolution limit [Å]	2.750	2.750
Rmerge		0.483
Number of reflections	32586
<I/σ(I)>	33.3
Completeness [%]	99.6
Redundancy	6.4
CC(1/2)	0.983	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Magnesium Acetate, 2-(N-morpholino)ethanesulfonic, 2-methyl-2,4-pentanediol