5ZEJ
Crystal structure of the bacterial A1408me1A-mutant ribosomal decoding site in complex with paromomycin
Replaces: 4WCRExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 31.592, 90.840, 46.812 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.598 - 3.500 |
| R-factor | 0.2076 |
| Rwork | 0.205 |
| R-free | 0.22820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3td1 |
| RMSD bond length | 0.058 |
| RMSD bond angle | 0.998 |
| Data reduction software | d*TREK |
| Data scaling software | CrystalClear |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.600 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.143 | 0.487 |
| Number of reflections | 1848 | |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 97.2 | |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium cacodylate, Spermine, MPD, Ammonium chloride |
