5ZEI
Crystal structure of the bacterial A1408me1A-mutant ribosomal decoding site in complex with geneticin
Replaces: 4WCQExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 33.430, 89.780, 46.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.900 - 2.100 |
| R-factor | 0.2061 |
| Rwork | 0.201 |
| R-free | 0.25010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3td1 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.621 |
| Data reduction software | d*TREK |
| Data scaling software | CrystalClear |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.900 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.059 | 0.365 |
| Number of reflections | 8419 | |
| <I/σ(I)> | 15.4 | |
| Completeness [%] | 96.6 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Sodium cacodylate, Spermine, MPD, ammonium chloride |
