5ZDM
The ligand-free structure of FomD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.474, 46.215, 52.504 |
| Unit cell angles | 90.00, 110.27, 90.00 |
Refinement procedure
| Resolution | 49.250 - 1.380 |
| R-factor | 0.16486 |
| Rwork | 0.164 |
| R-free | 0.19178 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3exm |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.384 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.410 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.031 | 0.399 |
| Number of reflections | 36195 | 1840 |
| <I/σ(I)> | 18 | 2.4 |
| Completeness [%] | 96.4 | 94.3 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 278 | Tris-HCl, calcium chloride, PEG 8000, trimethylamine N-oxide, cytosine |
