5Z98

Crystal Structure of the Primate APOBEC3H Dimer mediated by RNA Duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	38.341, 86.970, 77.363
Unit cell angles	90.00, 102.63, 90.00

Refinement procedure

Resolution	43.485 - 2.200
R-factor	0.2545
Rwork	0.251
R-free	0.32020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w3v
RMSD bond length	0.008
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.490	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.073	0.471
Number of reflections	24557	2074
<I/σ(I)>	8.5	2.1
Completeness [%]	97.3	94.7
Redundancy	3.1	2.8
CC(1/2)	0.996	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% PEG 3350, 100mM ammonium formate