5Z8L
crystal structure of Arabidopsis thaliana EBS in complex with an H3K27me3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.2827 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.247, 60.299, 98.038 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.019 - 2.005 |
| R-factor | 0.2026 |
| Rwork | 0.201 |
| R-free | 0.23010 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.203 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.103 | 0.797 |
| Number of reflections | 14980 | |
| <I/σ(I)> | 51 | 5.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.6 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2M sodium acetate, 20% PEG 3350 |
