5Z6W
Crystal structure of paFAN1 bound to 2nt 5'flap DNA with gap with Manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.874, 104.969, 105.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.990 - 3.200 |
R-factor | 0.185 |
Rwork | 0.182 |
R-free | 0.23760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r8a |
RMSD bond length | 0.013 |
RMSD bond angle | 1.155 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1-2155_1069: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.260 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.981 | 0.981 |
Rpim | 0.042 | 0.414 |
Number of reflections | 15512 | 762 |
<I/σ(I)> | 16.19 | |
Completeness [%] | 99.9 | |
Redundancy | 6.4 | |
CC(1/2) | 0.998 | 0.651 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 0.1M HEPES(pH7.2), 25%(w/v) PEG 8000, 30mM Gly-gly-gly |