5Z68
Structure of the recombination mediator protein RecF-ATP in RecFOR pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.005 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 108.118, 138.821, 179.567 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.863 - 3.000 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.25470 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.442 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| <I/σ(I)> | 28.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.8M ammonium sulfate, 100 mM Tris-Hcl, pH 8.5 |
