5Z5E
Crystal structure of the Glycyl-tRNA synthetase (GlyRS) in Nanoarchaeum equitans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 93.15 |
Detector technology | PIXEL |
Collection date | 2012-10-30 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.00000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.410, 121.096, 133.635 |
Unit cell angles | 90.00, 93.27, 90.00 |
Refinement procedure
Resolution | 48.198 - 2.098 |
R-factor | 0.2029 |
Rwork | 0.201 |
R-free | 0.24250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ati |
RMSD bond length | 0.007 |
RMSD bond angle | 0.839 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.098 | 2.098 |
Rmerge | 0.070 | 0.323 |
Number of reflections | 70618 | |
<I/σ(I)> | 13.1 | |
Completeness [%] | 95.9 | 88.4 |
Redundancy | 3.3 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277.15 | 0.05M sodium acetate trihydrate (pH 4.6), 1M ammonium sulfate and 3mg/ml protein |