5Z5A

Crystal structure of Tk-PTP in the active form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	CCD
Collection date	2017-06-15
Detector	ADSC QUANTUM 270
Wavelength(s)	0.987
Spacegroup name	P 61
Unit cell lengths	102.722, 102.722, 83.805
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.960 - 1.800
R-factor	0.176
Rwork	0.174
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5z59
RMSD bond length	0.008
RMSD bond angle	0.964
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.799	1.800
Number of reflections	45504
<I/σ(I)>	31.6
Completeness [%]	97.6
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.03M citric acid, 0.07M Bis-Tris propane (pH 7.6), 8%(w/v) polyethylene glycol 3350, 0.08M spermine tetrahydrochloride