5Z4U
Crystal Structure of T2R-TTL complex with 7a3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.491, 158.233, 182.099 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 119.440 - 3.180 |
| R-factor | 0.19049 |
| Rwork | 0.188 |
| R-free | 0.23515 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o2b |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.258 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 120.000 | 3.260 |
| High resolution limit [Å] | 3.180 | 3.200 |
| Number of reflections | 50942 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 98.7 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M Mes, 30 mM CaCl2, 30 mM MgCl2 (pH 6.7) |
