5Z44
Crystal structure of prenyltransferase AmbP1 complexed with GSPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-09 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.1000 |
Spacegroup name | P 41 |
Unit cell lengths | 115.717, 115.717, 48.431 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.676 - 2.458 |
R-factor | 0.1905 |
Rwork | 0.185 |
R-free | 0.24550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5z43 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.968 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.400 | 2.560 |
High resolution limit [Å] | 2.458 | 2.460 |
Rmerge | 0.039 | 0.413 |
Number of reflections | 23647 | 2713 |
<I/σ(I)> | 17.3 | 2.3 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 3.5 | 3.5 |
CC(1/2) | 0.999 | 0.867 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM MES (pH6.5), 0.2M MgCl2, 22% PEG8000 |