5Z3M
Crystal structure of Low Molecular Weight Phosphotyrosine phosphatase (VcLMWPTP-2) from Vibrio choleraeO395
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.912, 51.867, 83.896 |
Unit cell angles | 90.00, 103.62, 90.00 |
Refinement procedure
Resolution | 19.402 - 2.600 |
R-factor | 0.2538 |
Rwork | 0.252 |
R-free | 0.28830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lrq |
RMSD bond length | 0.005 |
RMSD bond angle | 1.061 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.402 | 2.692 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 31285 | |
<I/σ(I)> | 8 | |
Completeness [%] | 98.9 | |
Redundancy | 2.53 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | AMS, HEPES, GLYCEROL, MOPS, NaCl |