5Z30
The crystal structure of the nucleosome containing a cancer-associated histone H2A.Z R80C mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.399, 108.332, 170.902 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.700 - 2.450 |
| R-factor | 0.2036 |
| Rwork | 0.202 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wa9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.530 |
| High resolution limit [Å] | 2.440 | 5.250 | 2.440 |
| Rmerge | 0.092 | 0.070 | 0.415 |
| Rmeas | 0.101 | 0.077 | 0.488 |
| Rpim | 0.040 | 0.031 | 0.250 |
| Total number of observations | 336465 | ||
| Number of reflections | 66632 | 7117 | 6193 |
| <I/σ(I)> | 8.2 | ||
| Completeness [%] | 97.8 | 99.2 | 92.1 |
| Redundancy | 5 | 6.2 | 3 |
| CC(1/2) | 0.997 | 0.577 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride |
