5Z2T
Crystal structure of DNA-bound DUX4-HD2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-15 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.9747 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 51.610, 51.610, 166.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.297 - 2.623 |
R-factor | 0.2779 |
Rwork | 0.276 |
R-free | 0.29940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5z2s |
RMSD bond length | 0.005 |
RMSD bond angle | 0.826 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.300 | 2.760 |
High resolution limit [Å] | 2.620 | 2.620 |
Number of reflections | 7363 | |
<I/σ(I)> | 11.5 | |
Completeness [%] | 99.9 | |
Redundancy | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 273 | PEG 8000 |