5Z2S

Crystal structure of DUX4-HD2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-16
Detector	MAR CCD 130 mm
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	25.150, 25.390, 72.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.966 - 1.500
R-factor	0.19
Rwork	0.189
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a02
RMSD bond length	0.010
RMSD bond angle	1.127
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.300	1.580
High resolution limit [Å]	1.500	1.500
Number of reflections	7483
<I/σ(I)>	19.2
Completeness [%]	100.0
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		273	PEG 4000