5Z2F

NADPH/PDA bound Dihydrodipicolinate reductase from Paenisporosarcina sp. TG-14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	200
Detector technology	CCD
Collection date	2017-09-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 42 21 2
Unit cell lengths	79.942, 79.942, 83.835
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.100
R-factor	0.19021
Rwork	0.187
R-free	0.25657
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.807
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.150
High resolution limit [Å]	2.100	2.100
Number of reflections	16378
<I/σ(I)>	40.9
Completeness [%]	99.3
Redundancy	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Ammonium Sulfate, 0.1M Tris:HCl pH 8.5, and 25%(w/v) PEG 3350