5Z1Z
The apo-structure of D-lactate dehydrogenase from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-09 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 139.600, 150.490, 62.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.240 - 1.970 |
R-factor | 0.20509 |
Rwork | 0.204 |
R-free | 0.22735 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j49 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.698 |
Data scaling software | SCALA |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.240 | 2.080 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.089 | 0.599 |
Rpim | 0.039 | 0.325 |
Number of reflections | 90771 | 11859 |
<I/σ(I)> | 11.8 | 2.2 |
Completeness [%] | 98.6 | 88.2 |
Redundancy | 5.2 | 3.7 |
CC(1/2) | 0.997 | 0.597 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG 3350, 200 mM magnesium acetate |