5Z1I
Crystal structure of the protozoal cytoplasmic ribosomal decoding site in complex with 6'-fluoro sisomicin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-06 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 46.171, 46.171, 115.294 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.862 - 1.903 |
Rwork | 0.196 |
R-free | 0.22400 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.020 |
Data scaling software | XSCALE |
Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.862 | 42.862 | 1.950 |
High resolution limit [Å] | 1.900 | 8.510 | 1.900 |
Rmerge | 0.072 | 0.030 | 0.275 |
Rmeas | 0.075 | 0.032 | 0.288 |
Number of reflections | 5236 | 82 | 374 |
<I/σ(I)> | 23.85 | 45.26 | 7.86 |
Completeness [%] | 99.8 | 97.6 | 99.2 |
Redundancy | 11.955 | 9.427 | 11.481 |
CC(1/2) | 0.999 | 0.999 | 0.987 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2-Methyl-2,4-pentanediol, LiCl, spermine tetrahydrochloride, sodium cacodylate |