5Z1I

Crystal structure of the protozoal cytoplasmic ribosomal decoding site in complex with 6'-fluoro sisomicin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-06
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	I 4 2 2
Unit cell lengths	46.171, 46.171, 115.294
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.862 - 1.903
Rwork	0.196
R-free	0.22400
RMSD bond length	0.006
RMSD bond angle	1.020
Data scaling software	XSCALE
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.862	42.862	1.950
High resolution limit [Å]	1.900	8.510	1.900
Rmerge	0.072	0.030	0.275
Rmeas	0.075	0.032	0.288
Number of reflections	5236	82	374
<I/σ(I)>	23.85	45.26	7.86
Completeness [%]	99.8	97.6	99.2
Redundancy	11.955	9.427	11.481
CC(1/2)	0.999	0.999	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	2-Methyl-2,4-pentanediol, LiCl, spermine tetrahydrochloride, sodium cacodylate