5YYA

Crystal structure of Tandem Tudor Domain of human UHRF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-07-17
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	38.790, 60.004, 68.903
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.452 - 1.700
R-factor	0.1736
Rwork	0.172
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3db3
RMSD bond length	0.004
RMSD bond angle	0.626
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.250	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	17949
<I/σ(I)>	6.5
Completeness [%]	97.9
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1 M Bis-Tris (pH 6.5), 200 mM ammonium acetate, 25% (w/v) polyethylene glycol (PEG) 3350