5YWS
Crystal structure of TREX1 in complex with a Y structured DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2012-07-06 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.532, 96.604, 101.112 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.330 - 2.000 |
R-factor | 0.192 |
Rwork | 0.189 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mxm |
RMSD bond length | 0.004 |
RMSD bond angle | 0.851 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.330 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.074 | 0.487 |
Number of reflections | 39153 | |
<I/σ(I)> | 31.4 | 3.8 |
Completeness [%] | 97.4 | 100 |
Redundancy | 6.6 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1M SODIUM ACETATE TRIHYDRATE PH 4.5, 30%(V/V) POLYETHYLENE GLYCOL 300, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |