5YST
RanM189D in complex with RanBP1-CRM1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 104.884, 104.884, 307.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.040 |
| R-factor | 0.2106 |
| Rwork | 0.209 |
| R-free | 0.23770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hat |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.114 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.080 |
| High resolution limit [Å] | 2.040 | 5.540 | 2.040 |
| Rmerge | 0.156 | 0.102 | |
| Rmeas | 0.163 | 0.107 | |
| Rpim | 0.058 | 0.031 | 0.766 |
| Number of reflections | 110107 | 5999 | 5417 |
| <I/σ(I)> | 3 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 12.9 | 12.4 | 13.1 |
| CC(1/2) | 0.994 | 0.303 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 18% PEG 3350, 200mM Ammonium Nitrate, 100mM Bis-Tris pH 6.6 |
