5YSL
Crystal structure of antibody 1H1 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.183, 81.091, 98.472 |
| Unit cell angles | 85.68, 82.85, 71.45 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.21552 |
| Rwork | 0.212 |
| R-free | 0.27337 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sy6 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.787 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 64042 | |
| <I/σ(I)> | 13.326 | |
| Completeness [%] | 98.3 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 291.15 | 0.2 M potassium sulfate, 20% PEG 3350 |
