5YS3

1.8 angstrom crystal structure of Succinate-Acetate Permease from Citrobacter koseri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 42
Unit cell lengths	79.456, 79.456, 89.099
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.728 - 1.823
R-factor	0.1619
Rwork	0.160
R-free	0.19850
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	1.129
Data reduction software	HKL-2000 (v1.0)
Data scaling software	HKL-2000 (v1.0)
Phasing software	SHELXD (v1.0)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge		0.800
Number of reflections	51162
<I/σ(I)>	17.5	1.9
Completeness [%]	99.6	97.6
Redundancy	6.9	6.6
CC(1/2)		0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.5	298	PEG 400, CaAc2, NaCl